Vol. 56, No. 6, 2022

Molecular Biology

Virus Genome Editing

Editing of Phage Genomes—Recombineering-assisted SpCas9 Modification of Model Coliphages T7, T5, and T3

A. Isaev, A. Andriianov, E. Znobishcheva, E. Zorin, N. Morozova and K. Severinov p. 801 abstract

Antiviral Activity of CRISPR/Cas9 Ribonucleoprotein Complexes on a Hepatitis B Virus Model In Vivo

A. P. Kostyusheva, S. A. Brezgin, N. I. Ponomareva, I. A. Goptar, A. V. Nikiforova, V. I. Gegechkori, V. B. Poluektova, K. A. Turkadze, A. E. Sudina, V. P. Chulanov and D. S. Kostyushev p. 816 abstract

Bacterial Genome Editing

CRISPR Interference in Regulation of Bacterial Gene Expression

N. I. Nadolinskaia and A. V. Goncharenko p. 823 abstract

Genetic Engineering in Mycobacteria

D. K. Armianinova, D. S. Karpov, M. S. Kotliarova and A. V. Goncharenko p. 830 abstract

Repair of Double-Stranded DNA Breaks Generated by CRISPR–Cas9 in Pseudomonas putida KT2440

N. Sharaev, L. Chacon-Machado, O. Musharova, E. Savitskaya and K. Severinov p. 842 abstract

New Genome Editors

Prokaryotic Argonaute Proteins as a Tool for Biotechnology

E. V. Kropocheva, L. A. Lisitskaya, A. A. Agapov, A. A. Musabirov, A. V. Kulbachinskiy and D. M. Esyunina p. 854 abstract

Yeast Genome Editing

A Test System for Assessment of the Activity of Mutant Cas9 Variants in Saccharomyces cerevisiae

D. S. Spasskaya, A. I. Davletshin, V. V. Tutyaeva, K. A. Kulagin, D. G. Garbuz and D. S. Karpov p. 874 abstract

Plant Genome Editing

Genome Editing in Species of the Tribe Triticeae with the CRISPR/Cas System

B. R. Kuluev, E. V. Mikhailova, A. R. Kuluev, A. A. Galimova, E. A. Zaikina and E. K. Khlestkina p. 885 abstract

cis-Prenyltransferases of Marchantia polymorpha: Phylogenetic Analysis and Perspectives for Use as Regulators of Antimicrobial Agent Synthesis

L. R. Valeeva, S. M. Dzhabrailova and M. R. Sharipova p. 902 abstract

Worm Genome Editing

Transgenesis in Worms: Candidates for an Ideal Model

I. S. Sukhikh, M. Yu. Biryukov and A. G. Blinov p. 915 abstract

Mammalian Genome Editing

Genome Editing in Therapy of Genodermatoses

A. V. Ivanenko, N. A. Evtushenko and N. G. Gurskaya p. 921 abstract

The Efficiency of Gene Activation Using CRISPR/dCas9-Based Transactivation Systems Depends on the System Run Time

A. S. Artyuhov, D. A. Dorovskiy, A. V. Sorokina, K. M. Shakirova, E. D. Momotyuk and E. B. Dashinimaev p. 942 abstract

Structural Elements of DNA and RNA Eukaryotic Expression Vectors for In Vitro and In Vivo Genome Editor Delivery

A. A. Zagoskin, M. V. Zakharova and M. O. Nagornykh p. 950 abstract

Reviews

Prospects of Genetically Encoded Flim Indicators for the Quantitative Assessment of Intracellular Parameters

A. V. Mamontova, T. R. Simonyan and A. M. Bogdanov p. 963 abstract

Protein Homeostasis Dysregulation in Pathogenesis of Neurodegenerative Diseases

M. S. Kukharsky, M. W. Everett, O. A. Lytkina, M. A. Raspopova, E. A. Kovrazhkina, R. K. Ovchinnikov, A. I. Antohin and A. A. Moskovtsev p. 967 abstract

The p53 Tumor Suppressor and Copper Metabolism: An Unrevealed but Important Link

S. A. Tsymbal, A. G. Refeld and O. A. Kuchur p. 979 abstract

Genomics. Transcriptomics

Methylation Status of Apoptosis Genes and Intensity of Apoptotic Death of Peripheral Blood Lymphocytes in Persons Chronically Exposed to Radiation

E. A. Blinova, V. S. Nikiforov, A. I. Kotikova, M. A. Yanishevskaya and A. V. Akleyev p. 993 abstract

Genetic and Molecular Characterization of a Dash Cryptochrome Homologous Gene from Antarctic Diatom Phaeodactylum tricornutum ICE-H

M. L. An and J. L. Miao p. 1003 abstract

Cell Molecular Biology

Mechanism of Leakage in Phosphatidylserine-Containing Membranes by Melittin

R. Wang, M.-Y. Shi, B.-B. Ma and J. Sheng p. 1012 abstract

Blood–Brain Barrier Transwell Modeling

A. V. Petrovskaya, E. P. Barykin, A. M. Tverskoi, K. B. Varshavskaya, V. A. Mitkevich, I. Yu. Petrushanko and A. A. Makarov p. 1020 abstract

High Heterogeneity of Virus-Neutralizing and RBD-Binding Activities of COVID-19 Convalescent Sera

E. A. Astakhova, M. G. Byazrova, G. M. Yusubalieva, V. F. Larichev, V. P. Baklaushev and A. V. Filatov p. 1028 abstract

Full and D-BOX-Deficient PTTG1 Isoforms: Effects on Cell Proliferation

D. E. Demin, E. M. Stasevich, M. M. Murashko, E. A. Tkachenko, A. N. Uvarova and A. M. Schwartz p. 1036 abstract

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