Contents

Biochemistry (Moscow)

Vol. 88, No. 9, 2023

REVIEW

Recent Developments in Bioprocessing of Recombinant Antibody Fragments

Nevena Zelenovic, Lidija Filipovic, and Milica Popovic p. 1191  abstract

Novel B-Cell Epitopes of Non-Neutralizing Antibodies in the Receptor-Binding Domain of the SARS-CoV-2 S-Protein with Different Effects on the Severity of COVID-19

Andrey L. Matveev, Oleg V. Pyankov, Yana A. Khlusevich, Olga V. Tyazhelkova, Ljudmila A. Emelyanova, Anna M. Timofeeva, Andrey V. Shipovalov, Anton V. Chechushkov, Natalia S. Zaitseva, Gleb A. Kudrov, Gaukhar M. Yusubalieva, Saule M. Yussubaliyeva, Oxana A. Zhukova, Artem Yu. Tikunov, Vladimir P. Baklaushev, Sergey E. Sedykh, Galina I. Lifshits, and Nina V. Tikunova p. 1205  abstract

State-of-the-Art Approaches to Heterologous Expression of Bispecific Antibodies Targeting Solid Tumors

Aleksei K. Misorin, Darya O. Chernyshova, and Mikhail S. Karbyshev p. 1215  abstract

Non-Immunoglobulin Synthetic Binding Proteins for Oncology

Temitope I. David, Nikolay B. Pestov, Tatyana V. Korneenko, and Nikolai A. Barlev p. 1232  abstract

Heterologous Expression of Recombinant Proteins and Their Derivatives Used as Carriers for Conjugate Vaccines

Yuliya A. Khodak p. 1248  abstract

Generation of a Cell Line Selectively Producing Functionally Active OATP1B1 Transporter

Mariia S. Kotliarova, Aleksei V. Shchulkin, Pelageya D. Erokhina, Pavel Y. Mylnikov, Elena N. Yakusheva, Nonna I. Nadolinskaia, Mikhail V. Zamakhaev, and Anna V. Goncharenko p. 1267  abstract

Dimeric ACE2-FC Is Equivalent to Monomeric ACE2 in the Surrogate Virus Neutralization Test

Denis E. Kolesov, Elizaveta A. Gaiamova, Nadezhda A. Orlova, and Ivan I. Vorobiev p. 1274  abstract

Analysis of Some Biochemical Properties of Recombinant Siberian Roe Deer (Capreolus pygargus) Chymosin Obtained in the Mammalian Cell Culture (CHO-K1)

Denis E. Murashkin, Svetlana V. Belenkaya, Aleksandr A. Bondar, Vadim V. Elchaninov, and Dmitrii N. Shcherbakov p. 1284  abstract

Reconstitution of Calcium Channel Protein Orai3 into Liposomes for Functional Studies

Chuangxuan Liang, and Fuyun Wu p. 1296  abstract

Development of Heterologous Expression System and Optimization of the Method of Cholera Toxin β-Subunit Production in E. coli

Hamesd H. Jamgochian, Mikhail V. Zamakhaev, Nikolai N. Sluchanko, Anna V. Goncharenko, and Mikhail S. Shumkov p. 1304  abstract

Upregulation of YciM Expression Reduces Endotoxin Contamination of Recombinant Proteins Produced in Escherichia coli Cells

Pavel A. Bobrovsky, Daria D. Kharlampieva, Sergey A. Kirillin, Ksenia A. Brovina, Ekaterina N. Grafskaia, Vassili N. Lazarev, and Valentin A. Manuvera p. 1318  abstract

Location and Orientation of the Genetic Toxin–Antitoxin Element hok/sok in the Plasmid Affect Expression of Pharmaceutically Significant Proteins in Bacterial Cells

Yulia A. Khodak, Rolan R. Shaifutdinov, Danila S. Khasanov, Nadezhda A. Orlova, and Ivan I. Vorobiev p. 1326  abstract

CRISPR/Cas9-Mediated Genome Editing of the Komagataella phaffii to Obtain a Phytase-Producer Markerless Strain

Artur A. Tkachenko, Larisa N. Borshchevskaya, Sergey P. Sineoky, and Tatiana L. Gordeeva p. 1338  abstract

Expression of Synthetic cyp102A1-LG23 Gene and Functional Analysis of Recombinant Cytochrome P450 BM3-LG23 in the Actinobacterium Mycolicibacterium smegmatis

Veronika Y. Poshekhontseva, Nikolai I. Strizhov, Mikhail V. Karpov, Vera M. Nikolaeva, Alexey V. Kazantsev, Olesya I. Sazonova, Andrey A. Shutov, and Marina V. Donova p. 1347  abstract

Production and Characterization of Photorin, a Novel Proteinaceous Protease Inhibitor from the Entomopathogenic Bacteria Photorhabdus laumondii

Igor M. Berdyshev, Anastasia O. Svetlova, Ksenia N. Chukhontseva, Maria A. Karaseva, Anna M. Varizhuk, Vasily V. Filatov, Sergey Y. Kleymenov, Sergey V. Kostrov, and Ilya V. Demidyuk p. 1356  abstract

Effect of Biotin Starvation on Gene Expression in Komagataella phaffii Cells

Anastasiya S. Makeeva, Anton V. Sidorin, Valeria V. Ishtuganova, Marina V. Padkina, and Andrey M. Rumyantsev p. 1368  abstract

NAD+-Dependent Formate Dehydrogenase from Themotolerant Yeast Ogataea parapolymorpha: Properties and Protein Engineering of the N-Terminal Sequence

Anastasia A. Pometun, Leonid A. Shaposhnikov, Sofiya A. Zubanova, Rostislav P. Kovalevskii, Denis L. Atroshenko, Evgenii V. Pometun, Svyatoslav S. Savin, and Vladimir I. Tishkov p. 1378  abstract

Ultra-Fast Mass Spectrometry in Plant Biochemistry: Response of Winter Wheat Proteomics to Pre-Sowing Treatment with Iron Compounds

Tomiris T. Kusainova, Daria D. Emekeeva, Elizaveta M. Kazakova, Vladimir A. Gorshkov, Frank Kjeldsen, Mikhail L. Kuskov, Alexey N. Zhigach, Irina P. Olkhovskaya, Olga A. Bogoslovskaya, Natalia N. Glushchenko, and Irina A. Tarasova p. 1390  abstract

Impaired Enzymatic Antioxidant Defense in Erythrocytes of Rats with Ammonia-Induced Encephalopathy: Role of NMDA Receptors

Elena A. Kosenko, Gubidat A. Alilova, and Lyudmila A. Tikhonova p. 1404  abstract