Contents

Moscow University Biological Sciences Bulletin

Vol. 80, No. 1, 2025

EDITORIAL

Results of the Fifth Russian International Conference on Cryo-Electron Microscopy (RICCEM-2025)

T. B. Stanishneva-Konovalova, I. A. Yaroshevich, M. P. Kirpichnikov, and O. S. Sokolova p. S1  abstract

REVIEW

Studying Flexible Elements of Microtubule Structure Using Cryo-Electron Microscopy and Molecular Modeling: Mission Possible?

V. A. Fedorov, E. G. Kholina, N. B. Gudimchuk, and I. B. Kovalenko p. S5  abstract

Overcoming the “Resolution Gap”: Combining Super-Resolution Microscopy and Cryo-Electron Tomography to Identify Mitochondrial Vimentin Binding Sites

I. B. Alieva, A. S. Shakhov, A. S. Churkina, and A. A. Minin p. S11  abstract

RESEARCH ARTICLE

The Morphology of Erythrocytes Reveals Differences in the Mechanisms of Erythrocyte-Derived Extracellular Vesicle Formation

E. Yu. Parshina, L. S. Frolova, and G. V. Maximov p. S18  abstract

Membrane-Associated Vesicles: Approaches for Isolation and Characterization

O. A. Grigorieva, N. A. Basalova, M. A. Vigovskiy, U. D. Dyachkova, D. V. Bagrov, O. S. Sokolova, and A. Yu. Efimenko p. S26  abstract

Cryo-Electron Microscopy Demonstrates Polymorphism of Small Extracellular Vesicles

T. S. Trifonova, A. V. Moiseenko, G. O. Skryabin, O. T. Imaraliev, E. M. Tchevkina, I. A. Karasev, A. Yu. Efimenko, S. I. Trifonov, D. V. Bagrov, and O. S. Sokolova p. S32  abstract

RESEARCH ARTICLE

N- or C-terminal Position of the Fluorescent Protein mKate2 in the mKate2-KCa3.1 Chimera Influences Membrane Expression of the Channel

V. N. Korabeynikova, A. V. Feofanov, and O. V. Nekrasova p. S39  abstract

Visualization of Artificial Polynucleosomal Constructs with Atomic Force Microscopy

A. V. Lyubitelev, D. V. Bagrov, O. V. Geraskina, and V. M. Studitsky p. S45  abstract

Sample Preparation of the Elongation Complex +39 for Cryo-Electron Microscopy

E. V. Osina, A. V. Moiseenko, A. N. Korovina, N. S. Gerasimova, O. I. Volokh, V. M. Studitsky, and T. B. Stanishneva-Konovalova p. S51  abstract

PARP1-Dependent Alterations in Nucleosome Organization: Potential Involvement of p53

D. O. Koshkina, N. V. Maluchenko, A. M. Novichkova, A. V. Feofanov, and V. M. Studitsky p. S59  abstract

Ni-NTA Bead-Based Real-Time Monitoring of PARPs Inhibitor Binding

A. A. Lobanova, A. A. Saulina, O. V. Geraskina, D. O. Koshkina, N. V. Maluchenko, A. V. Feofanov, and V. M. Studitsky p. S64  abstract

RESEARCH ARTICLE

Optimization of Recombinant Human Poly(ADP-ribose) Polymerase 3 Production

E. A. Volkova, A. N. Korovina, N. V. Maluchenko, and V. M. Studitsky p. S71  abstract

RESEARCH ARTICLE

Structural Features of Carotenoid-Binding Proteins

M. M. Surkov, A. Yu. Litovets, A. A. Mamchur, T. B. Stanishneva-Konovalova, and I. A. Yaroshevich p. S77  abstract

SHORT COMMUNICATION

Structure of the Translation Preinitiation Complex from Wheat Germ Extract

O. V. Kravchenko, Yu. M. Chesnokov, Zh. A. Afonina, and K. S. Vassilenko p. S85  abstract

Reconstruction of the phi24B Stx-Converting Bacteriophage Axial Fibril Using Cryo-Electron Microscopy

A. V. Moiseenko, M. A. Bubenchikov, Y. Zhang, A. S. Kuznetsov, A. V. Letarov, and O. S. Sokolova p. S89  abstract

SHORT COMMUNICATION

Study of a Cold-Adapted Attenuated SARS-CoV-2 Mutant Using Transmission, Cryo-Electron, and Atomic Force Microscopy

L. V. Kordyukova, A. V. Moiseenko, T. C. Trifonova, A. I. Akhmetova, A. V. Gracheva, E. R. Korchevaya, I. V. Yaminsky, and E. B. Faizuloev p. S93  abstract

SHORT COMMUNICATION

Structural Role of Pob3 CTD in FACT-Mediated Nucleosome Uncoiling Revealed by Electron Microscopy

O. I. Volokh, A. L. Sivkina, V. M. Studitsky, and O. S. Sokolova p. S99  abstract

SHORT COMMUNICATION

Analysis of Heteroсhromatin Mesoscale Architecture by Cryo-Electron Tomography

E. P. Kazakov, Y. M. Chesnokov, I. I. Kireev, and S. A. Golyshev p. S103  abstract

Critical Analysis of DNA Packaging Models at Different Types of Stress

Y. F. Krupyanskii, N. G. Loiko, V. V. Kovalenko, A. A. Generalova, E. V. Tereshkin, T. S. Trifonova, K. B. Tereshkina, G. S. Peters, and O. S. Sokolova p. S108  abstract