Contents

Applied Biochemistry and Microbiology

Vol. 61, No. 9, 2025

PROBLEMS AND PROSPECTS

3D-Cell Culture Systems: Current State

I. A. Ivashchenko, W. W. Wasielewski, O. S. Fedotova, V. N. Slautin, and A. V. Semenov p. 1645  abstract

PRODUCERS, BIOLOGY, SELECTION, AND GENETIC ENGINEERING

Semen Quality of Holstein Bulls and Expression of the AQP7, STK35, ITF27, and PRM1 Genes: Correlation Analysis

O. Yu. Barkova, D. A. Starikova, I. V. Chistyakova, and N. V. Pleshanov p. 1657  abstract

Peculiarities of Cryopreservation and Decryopreservation of Primordial Germ Cells in the Pushkin Breed of Chickens

G. K. Peglivanyan, T. A. Larkina, N. R. Reinbach, E. A. Polteva, A. P. Dysin, A. E. Ryabova, A. I. Azovtseva, and A. A. Krutikova p. 1664  abstract

Comparative Genetic Analysis of Pectinase Genes PGU of the Yeast Saccharomyces cerevisiae: Selection of Strains with High Pectinolytic Activity

A. N. Borovkova, M. Yu. Shalamitskiy, and E. S. Naumova p. 1669  abstract

The Effect of Deletion of the NDE1 Gene on Properties of the Yeast Schizosaccharomyces pombe Producing L-Lactic Acid

E. O. Anisimova, D. D. Bocharov, S. P. Sineoky, and M. G. Tarutina p. 1679  abstract

The Search for New Rice Genotypes with Resistance to Deep-Sea Flooding

N. G. Chertkova, P. I. Kostylev, A. V. Usatov, N. G. Dupliy, and E. A. Faddeeva p. 1689  abstract

Selection of the Optimal Medium Composition and Cultivation Conditions for the Maximum Yield of Rhamnolipids from Heterologous Producers

M. N. Baramzin and S. G. Litvinets p. 1696  abstract

A Collection of Recombinant Beta-Lactamases for Antibacterial Drug Screening

V. G. Grigorenko, O. V. Serova, I. P. Andreeva, M. Yu. Rubtsova, and A. M. Egorov p. 1704  abstract

BIOPREPARATION TECHNOLOGY

Development of Antiviral Drugs for Intranasal Administration Based on Double-Stranded RNA and Recombinant Interferons

O. S. Ivanova, G. M. Levagina, Y. V. Telegina, S. V. Usova, E. S. Bashkina, S. G. Gamaley, and E. D. Danilenko p. 1710  abstract

Cultivation of the CHO Cell Line in Bioreactors of Various Volumes: Technological Optimization

A. V. Kirilin, I. I. Tsvetkova, A. B. Sarbasov, P. Y. Romanova, M. A. Tsiruleva, E. A. Guzov, V. V. Sapovskaya, A. V. Erkhov, M. S. Afanasiev, A. V. Iserkapov, V. M. Kolyshkin, and V. G. Ignatyev p. 1719  abstract

Biodegradable Composite Materials Based on Natural Polymers

V. A. Demina, A. A. Stupnikov, P. M. Gotovtsev, S. V. Krashenninikov, and S. N. Chvalun p. 1726  abstract

MEDICAL BIOTECHNOLOGY

A New Relevant Cellular Model of Uterine Leiomyoma: Method of Isolation and Prospects of Use

N. Yu. Shved, A. A. Egorova, S. V. Shtykalova, N. S. Osinovskaya, I. Yu. Kogan, and A. V. Kiselev p. 1734  abstract

Evaluation of the Effectiveness of Fluorescent Labeling of Cells and Microvesicles with the Katushka2S Protein to Assess Their Biodistribution In Vivo and Ex Vivo

V. A. Kiryanova, A. M. Aimaletdinov, S. K. Kletukhina, and M. O. Gomzikova p. 1743  abstract

Optimization of Gas Permeability in PDMS Microfluidic Chips for Organ-on-Chip Modeling

I. A. Khaustov, Yu. A. Safronova, O. E. Chebotareva, J. A. Brianton, and E. N. Knyazev p. 1753  abstract

Downregulation of ELOVL5 Gene Expression Alters the Mechanism of Ferroptosis in MDA-MB-231 Breast Cancer Cells

A. V. Razumovskaya, T. A. Kulagin, A. A. Fatkulin, M. O. Silkina, D. M. Olkhovik, and S. V. Nikulin p. 1760  abstract

USE OF BIOPREPARATIONS

Aqueous Dispersions of Fullerene C60 and Fullerene Mixtures as Biopreparations for Stimulation of Plant Growth

O. V. Yamskova, D. V. Kurilov, O. A. Shchuklina, M. M. Ilyin, and A. A. Goryunkov p. 1770  abstract

The Effect of Treatment of Triticale with Growth Regulators of Verva and Ecopene on the Reproductive Ability of the Common Grass Aphid (Schizaphis graminum R.)

Ya. Yu. Golivanov and E. V. Zakharova p. 1780  abstract

SYSTEM ANALYSIS, MATHEMATICAL MODELING, INFORMATION SYSTEMS

During SARS-CoV-2 Evolution, the Interaction between Virus RNA and Host RNA-Binding Proteins Gradually Weakened

K. E. Malkova, A. P. Zhiyanov, A. V. Belaya, A. V. Arzumanova, I. D. Kirillov, and S. A. Tonevitskaya p. 1787  abstract

SYSTEM ANALYSIS, MATHEMATICAL MODELING, INFORMATION SYSTEMS

Monte Carlo Method for Calculating the Probability of Monoclonality of Cell Lines

S. S. Timonova, V. I. Pavelko, I. A. Kiric, V. N. Bade, A. A. Piskunov, and R. A. Khamitov p. 1794  abstract

ECOLOGY

The Influence of Antibiotic Ceftriaxone on the Black Soldier Fly (Hermetia illucens)

E. V. Mechtaeva, A. V. Zerov, V. V. Dzyubenko, P. N. Sorokoumov, O. V. Astafyeva, M. V. Novikova, A. Z. Zhuravleva, and V. Yu. Sitnov p. 1803  abstract

The Influence of Magnesium and Phosphorus Compounds on the Composition of the Effluent and Biogas Productivity during Anaerobic Fermentation of Manure from Laying Hens

I. V. Miroshnichenko, I. I. Vasilenko, V. A. Lomazov, D. N. Klyosov, and A. V. Lomazov p. 1811  abstract

METROLOGY, STANDARDIZATION, CONTROL

Selection and Substantiation of Methods for Estimating the Consumer Properties of Cosmetic Products by Deodorant and Antiperspirant Effect. Part 2. Development of an Express Method for Estimating the Intrinsic Antimicrobial Activity of the Products

Yu. A. Penkina, V. R. Normatova, T. T. N. Ha, and I. A. Butorova p. 1823  abstract

SHORT COMMUNICATIONS

Efficient and Sensitive Method for Detection of Transposon Insertions in Plants Based on Targeted Nanopore Amplicon Sequencing

P. Yu. Merkulov, M. A. Serganova, M. V. Dudnikov, and I. V. Kirov p. 1832  abstract

Erratum

Erratum to: Identification of the Causal Agent of Grapevine Downy Mildew Plasmopara viticola Based on Quantitative PCR

N. N. Nityagovsky, A. A. Dneprovskaya, A. A. Ananev, K. V. Kiselev, and O. A. Aleynova p. 1837  abstract

Erratum to: Rhodococcus erythropolis А-27 as a Biocatalyst for Enantioselective Reduction of Ketones in the Presence of High Concentrations of Isopropanol

N. I. Petukhova, A. V. Mityagina, and V. V. Zorin p. 1838  abstract

Erratum to: Ribosome Disorganization and Other Effects of Artifitial RNAse DL412 on Salmonella enterica Cells

A. E. Grigor’eva, A. V. Tupitsyna, E. S. Ryabova, A. V. Bardasheva, D. A. Zadvornykh, L. S. Koroleva, V. N. Silnikov, and E. I. Ryabchikova p. 1839  abstract

Erratum to: Effect of Fermentation with Different Lactobacilli on the Organoleptic Properties of Pea Protein Isolate

I. V. Kravchenko, V. A. Furalyov, E. S. Pshennikova, A. N. Fedorov, and V. O. Popov p. 1840  abstract

Erratum to: Probiotic Properties of Lactobacillus helveticus Isolated from Kefir Grains and Feces of Homo sapiens

O. S. Savinova, A. V. Shabaev, and T. V. Fedorova p. 1841  abstract

Erratum to: Biosynthesis of Suberiс Acid from Glucose through Inverted Fatty Acid β-Oxidation by Recombinant Escherichia coli Strains

A. Yu. Gulevich, A. Yu. Skorokhodova, and V. G. Debabov p. 1842  abstract

Erratum to: Synthesis and Antibacterial Activity of Silver Nanoparticles Stabilized by Lipopeptides and Glycolipids Produced by Bacillus amyloliquefaciens and Pseudomonas fluorescens

A. G. Khina, A. S. Gordeev, L. R. Biktasheva, D. M. Gorbunov, P. A. Kuryntseva, G. V. Lisichkin, and Yu. A. Krutyakov p. 1843  abstract

Erratum to: Degradation of Dibutyl Phthalate by the Halotolerant Strain Pseudarthrobacter sp. NKDBFgelt

O. V. Yastrebova, A. A. Pyankova, A. V. Nazarov, Yu. I. Nechaeva, E. S. Korsakova, and E. G. Plotnikova p. 1844  abstract

Erratum to: Optimization of 1,3-Butanediol Biosynthesis from Glucose through the Inverted Fatty Acid β-Oxidation Pathway by Recombinant Escherichia coli Strains

A. Yu. Gulevich, A. Yu. Skorokhodova, and V. G. Debabov p. 1845  abstract

Erratum to: Impact of Neurotransmitters on the Photosynthetic Pigment Content of the Green Microalga Haematococcus lacustris (Strains IPPAS H-239 and BM-1)

Cao Boyang, T. A. Fedorenko, O. B. Chivkunova, A. E. Solovchenko, E. S. Lobakova, and A. V. Oleskin p. 1846  abstract

Erratum to: Glycation of Leghemoglobin by Methylglyoxal in Comparison with Other Hemoglobins and the Influence on Peroxidase Activity

E. I. Nasybullina, O. V. Kosmachevskaya, and A. F. Topunov p. 1847  abstract