Antigen from Winter Rye Accumulated under Low Temperature

A. V. Kolesnichenko, G. B. Borovskii, V. K. Voinikov, S. I. Misharin, and A. I. Antipina

Siberian Institute of Plant Physiology and Biochemistry, Siberian Division, Russian Academy of Sciences,

ul. Lermontova 132, Irkutsk, 664033 Russia

Received December 12, 1995

Abstract—The low temperature–responsible antigen with R_f 0.35 was isolated and purified from rye (Secale
cereale L.) seedlings. This antigen was precipitated with ammonium sulfate at 30–50% saturation. Further puri-
fication was achieved by column chromatography on a Sephadex 9-100. The immunochemically pure antigen
was eluted by 0.05 M ammonium bicarbonate in the first peak. The mol wt of the native antigen was electro-
phoretically estimated as 310 kD; the antigen consisted of two subunits with mol wts of 66 and 56 kD. By appli-
cation of antiserum against the purified antigen, rapid (within an hour) antigen accumulation was demonstrated
in response to both above- and below-zero temperatures, with the most pronounced effect (by 50%) being
observed after seedling exposure to 3°C.

Key words: Secale cereale - low temperature - antigen - purification

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