Activities of Antioxidant Enzymes of Arabidopsis thaliana Plants during Cold Hardening1 to Hypothermia
M. S. Sin’kevicha, A. A. Selivanova, O. V. Antipinaa, E. V. Kropochevab, G. P. Alievaa, T. A. Suvorovaa, N. V. Astakhovaa, and I. E. Moshkova
Translated by A. Averyanov
a Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, 127276 Russia
b Moscow State University, Moscow, 119991 Russia
Correspondence to: e-mail: sinkevich_m@mail.ru
1Abbreviations: CAT—catalase; MDA—malonic dialdehyde; POL—peroxidation of lipids; POX—peroxidase; SOD—superoxide dismutase.
Received 5 November, 2015
Abstract—Changes in activities of the enzymes performing direct antioxidant functions were studied in 7–8-week-old plants Arabidopsis thaliana Heinh (L.) of Columbia (Col-0) ecotype. It was found that 5-day cold hardening at 2°C increased plant cold resistance to the subsequent stronger cooling. Under these conditions, the marked changes occurred in activities of superoxide dismutase and III type (guaiacol) peroxidses but not in that of catalase. The total peroxidase activity exceeded the catalase activity before cold hardening. Therefore, peroxidases are able to decompose more H2O2 than catalases and appear to make the dominant contribution to the protection from the cold damage.
Keywords: Arabidopsis thaliana, cold hardening, ROS, oxidative stress, superoxide dismutase, peroxidase, catalase
DOI: 10.1134/S1021443716060108