Synthesis of Silver Nanoparticles Modified with Lysozyme and Sodium Dioctyl Sulfosuccinate and Comparison of Their Biological Activities

Abstract—Silver nanoparticles have been fabricated by the method of chemical reduction in solution using different types of stabilizers: an organic low-molecular compound—sodium dioctyl sulfosuccinate (AOT)—and a natural biologically active substance—antimicrobial cationic polypeptide lysozyme. According to studies of the produced hydrosols, the average size of the shell-coated particles is 20–25 nm. The biological activity of the obtained bioconjugates toward Gram-negative (Escherichia coli ML35p, Pseudomonas aeruginosa (clinical isolate)) and Gram-positive (Listeria monocytogenes EGD (АТСС ВАА-679), MRSA ATCC 33591 (Staphylococcus aureus resistant to methicillin)) bacteria has been investigated by the methods of radial diffusion in an agarose gel and serial dilution in a liquid nutritional medium. It has been established that the antimicrobial activity of the bioconjugates depends on the nature of the used stabilizer.