The Effect of Silver Nanoparticles on the Photocycle of Bacteriorhodopsin of Purple Membranes of Halobacterium salinarum

V. A. Oleinikova,b, K. E. Mochalova,b, D. O. Solovyevaa,b, A. A. Chistyakova,b, E. P. Lukashevc, and I. R. Nabievb,dTranslated by E. Berezhnaya

a Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia

b National Research Nuclear University, Moscow Engineering Physics Institute, Moscow, 115409 Russia

c Moscow State University, Moscow, 119991 Russia

d University of Reims Champagne-Ardenne, Reims, 51100 France

Correspondence to: e-mail:

Received 24 February, 2016

Abstract—The effect of silver nanoparticles (AgNPs) that are adsorbed on the surface of the purple membranes of Halobacterium salinarium bacteria on the optical properties and functional peculiarities of the light-sensitive protein bacteriorhodopsin (BR) has been demonstrated for the first time. Two mechanisms of the effect of AgNPs on the protein photocycle have been demonstrated using Raman scattering, giant Raman scattering, flash photolysis, and atomic force microscopy. It has been shown that the nanoparticles in the immediate spatial vicinity of BR fix its photocycle at the stage where it was at the moment of interaction with the nanoparticles. At greater distances, which reach three radii of an AgNPs, they have a weaker effect on BR, under which it retains the ability to be involved in the photocycle, however, has its parameters significantly changed. Thus, in the case of wild-type BR the photocycle accelerates and for the BR-D96N mutant it becomes slower. The data that are obtained could be of significance for creation of such optoelectronic hybrid systems with BR, where the parameters of its photocycle can be controlled using NPs. The results of the study may also be used in the field of nanobioengineering research, which is directed to creation of unique materials with controlled properties for recording and storage of information, energy transformation, and identification and characterization of trace amounts of analytes.

DOI: 10.1134/S0030400X1608018X