Absorption Spectroscopy of Microalgae, Cyanobacteria,
and Dissolved Organic Matter: Measurements
in an Integrating Sphere Cavity

S. I. Pogosyan, A. M. Durgaryan, I. V. Konyukhov, O. B. Chivkunova, and M. N. Merzlyak

Moscow State University, Biological Department, Moscow, Russia

E-mail: pogosyan@biophys.msu.ru

Received July 6, 2008

Abstract—A device for integrating cavity absorption measurements (ICAM) with an internal diameter of
80mm suitable for field research is described. The spectral features of the light absorption by some cyanobac-
teria, green algae, and diatoms in the integrating sphere were studied and the dependences of the absorption on
the cell concentration were determined in comparison with the conventional measurements in a 1-cm cuvette.
The sensitivity of the chlorophyll estimation with the ICAM reached 0.2–0.5 mg m–3. The results of the ICAM
application for the direct analysis of the natural phytoplankton and dissolved organic (“yellow”) matter in the
Black Sea and the Sea of Japan are described.

DOI: 10.1134/S0001437009060125


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