Optimization of a Preparation of Lentiviral Particles
for Transduction of Neurons in vivo

G. R. Tukhbatova, E. P. Kuleshova, M. Yu. Stepanichev, A. D. Ivanov, and S. V. Salozhin¹

Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia

Received April 14, 2011

Abstract—The transduction of neurons using the lentiviral systems is a widely used method for the delivery of
genetic material to cells of the nervous system in vivo. In the present study, we optimized the conditions for the
preparation of lentiviral particles for injections to the CNS of experimental animals. We describe a method of
transfection of packaging cells with the use of polyethyleneimine. A higher transfection efficacy was observed
in the serum-free medium when the cell density level was 60% of the maximum and the
DNA:polyethyleneimine ratio was 1 : 1.5. These transfection conditions may be used for the preparation of a
suspension of lentiviral particles for the effective transduction of neurons in vivo.

Keywords: lentiviruses, transduction of neurons in vivo

DOI: 10.1134/S1819712411040179

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