Histochemical Study of Neurodegenerative and Regenerative Processes in Flexor and Extensor Collaterals
of the Sciatic Nerve after Crushing in the Presence of PRP-1
A. L. Minasyana, A. V. Aznauryana, I. B. Meliksetyanb, V. A. Chavushyanb,
J. S. Sarkissianb,1, and A. A. Galoyanc
aHeratsi Yerevan State Medical University, Yerevan, Armenia
bOrbeli Institute of Physiology, National Academy of Sciences, Yerevan, Armenia
cBuniatian Institute of Biochemistry, National Academy of Sciences, Yerevan, Armenia
Received February 18, 2011
Abstract—A comparative morphological and histochemical study of the dynamics of de- and regenerative pro-
cesses in the damaged sciatic nerve (SN) area was conducted in rats at different time points after crushing in
the presence of proline-rich peptide (PRP-1). We measured the activity of Ca2+-dependent acid phosphatase
(AP). At the fifth day after SN crushing on the site of injury, we observed changes in the directions of nerve
fibers and disruption of their structure, in particular, swelling of Schwann hollow tubes. Later, we found prolif-
eration of cells of the endoneurium and Schwann cells in the extensor bundle (N. peroneus communis), which
promoted recovery of nerve fibers and was accompanied by appearance of the maximum activity AP. We also
showed partial recovery of the flexor bundle (N. Gastrocnemius). At 17 days after the trauma, as a result of suc-
cessful regeneration of the damaged nerve, we observed the recovery of nerve fibers and their structures, which
confirms the neuroprotective characteristics of PRP-1
Keywords: nerve crushing, extensor and flexcor collaterals of sciatic nerve, revealing of Ca2+-dependent acidic
phosphatase activity, PRP-1
DOI: 10.1134/S181971241104012X
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