G. F. Sitdikovaa,1, A. V. Yakovleva, Y. G. Odnoshivkinab, and A. L. Zefirovb
aKazan University, ul. Kremlevskaya 18, Kazan, Russia
bKazan Medical University, ul. Butlerova 49, Kazan, Russia
Received January 16, 2011
Abstract—Using electrophysiological and optical methods, we studied the effects of sodium hydrosulfide
(NaHS), a hydrogen sulfide donor, on the dynamics of transmitter release and exo- and endocytosis of synaptic
vesicles in motor nerve endings during long-term high-frequency stimulation (20 Hz) in experiments with the
cutaneous pectoris frog muscle. H2S increased the amplitude of endplate currents under conditions of a single
stimulation of the motor nerve and slowed down the depression of the end plate currents during high-frequency
stimulation (20 Hz, 3 min). Using the endocytic fluorescent dye FM 1-43, we showed that NaHS increased the
dye uptake during high-frequency stimulation as compared to the control. However, after termination of the
high-frequency stimulation the fluorescence intensity of nerve terminals was lower in the presence of NaHS
than in the control experiments. In addition, NaHS slowed the dye release from the nerve terminals that were
loaded during 20 Hz stimulation. The results thus obtained indicate that H2S accelerates the synaptic vesicles
cycle in frog motor nerve ending by enhancement of exocytosis and fast endocytosis of synaptic vesicles during
high-frequency stimulation.
Keywords: hydrogen sulfide, transmitter release, motor nerve ending, end-plate currents, endo- and exocytosis,
synaptic vesicles, FM 1-43
DOI: 10.1134/S1819712411040155
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