Structure and Permeability Changes of the BloodBrain Barrier
in APP/PS1 Mice: an Alzheimers Disease Animal Model

Yulin Wang^a, Jing Liu^a, Zhongmin Zhang^b, Xiaoli Wang^c, Chaodong Zhang^a,1

^aDepartment of Neurology, The first affiliated hospital, China Medical University, Shen Yang, China

^bDepartment of Neurology, Hong Qi Hospital, Mu Dan Jiang Medical College, Mu Dan Jiang, China

^cDepartment of Aging diseases, The second affiliated hospital, Mu Dan Jiang Medical College, Mu Dan Jiang, China

Abstract—The present work explored the blood–brain barrier (BBB) changes in a mice model of Alzheimer’s
disease (AD) (APP/PS1 double mutant). The transgenic mice model showed overexpression of A1-42 in the
brain and the loss of learning functions. The test with tail vein injection of Evans Blue (EB) showed more EB
fluorescence in the brain tissue, suggesting an increased BBB permeability. Additionally, Electronic micro-
scope (EM) images showed changes in base membrane, vessel-associated glial cells, pericytes, and endothelial
cells. This work supported the BBB dysfunction hypothesis in AD, and suggested that APP/PS1 mice could be
a suitable model in testing BBB-crossing drugs of AD.

Keywords: Alzheimer’s disease; blood–brain barrier; electronic microscopy; Evans blue; APP/PS1; transgenic

DOI: 10.1134/S1819712411030135

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