D. I. Pereguda,c,1, A. A. Yakovleva,b,c, L. F. Panchenkoa,c, and N. V. Gulyaevab
aNational Research Center on Addiction, Russian Ministry of Health and Social Development,
Moscow, Russia
bInstitute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia
cInstitute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia
Received October 28, 2010
Abstract—The expression of specific genes is an important factor of neuroplastic changes during the formation
of opiate dependence. Neurotrophic factors participate in structural-functional modifications in CNS regions after
opiate intoxication. Here, we studied the levels of mRNAs of the brain-derived neurotrophic factor (BDNF), nerve
growth factor (NGF), and insulin-like growth factor 1 (IGF1) in the frontal cortex, striatum, hippocampus, and
midbrain after spontaneous morphine withdrawal in dependent rats. To induce physical dependence, morphine
was injected intraperitoneally twice a day with increasing doses of 10–100 mg/kg for 6days. The expression of
mRNAs of BDNF, NGF, and IGF1 in the brain areas was estimated 40 hours after spontaneous morphine with-
drawal using the real-time PCR method. We found that spontaneous morphine withdrawal induced an elevation of
BDNF and IGF1 mRNAs in the frontal cortex. In the hippocampus and the midbrain only BDNF mRNA
increased. The content of NGF mRNA did not change in all regions studied. We believe that changes in the expres-
sion of BDNF and IGF1 are involved in the mechanisms of neuroplastic modification during the formation of opi-
ate dependence.
Keywords: mRNA, neurotrophic factors, brain, morphine, dependence, spontaneous withdrawal
DOI: 10.1134/S181971241102005X
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