E. V. Novosadova, E. L. Arsen’eva, A. G. Kobylyanskii, A. N. Lebedev, E. S. Manuilova,
V. Z. Tarantul, N. V. Khaidarova, and I. A. Grivennikov1
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia
Received September 10, 2010
Abstract—We studied the effect of overexpression of the human pub gene on the proliferation and neuronal
differentiation of PC-12 cells treated with nerve growth factor. We obtained a stable cell line overexpressing the
human pub gene (the PC12-hpub line) and PC12-DNA3 control cell line. Using reverse transcription PCR, we
showed transgene expression in cells of the PC12-hpub line. We found that under standard culturing conditions
and under conditions of deprivation of trophic factors (1% embryonic calf serum), the growth rate of PC12-
hpub cells did not differ from the rate of control PC12-DNA3 cells. After the induction of differentiation in
these cells lines using nerve growth factor in the absence of serum, we found that, in 5 days, the number of dif-
ferentiated cells with the neuronal phenotype in the PC12-hpub cells was smaller by a factor of 5 as compared
to the control PC12-DNA3 cells. Thus, overexpression of the human pub gene in rat pheochromocytoma PC12
cells led to the suppression of their nerve-growth-factor-induced differentiation.
Keywords: rat pheochromocytoma PC12 cells, transfection, proliferation, differentiation, polymerase chain
reaction, gene pub, nerve growth factor (NGF)
DOI: 10.1134/S1819712411010065
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