A. L. Minasyana, A. V. Aznauryana, I. B. Meliksetyanb, V. A. Chavushyanb, and J. S. Sarkissianb,1
aHeratsi Yerevan State Medical University, Yerevan, Armenia
bOrbeli Institute of Physiology, National Academy of Sciences, Yerevan, Armenia
Received June 2, 2010
Abstract—We performed morphological, histochemical, and electrophysiological analysis of changes in the
state of the rat sciatic nerve after nerve crushing. The activity of Ca2+-dependent acidic phosphatase (AP) was
measured in sections of the nerve fibers to trace the recovery of the nerve after its injury. The systemic applica-
tion of parathyroid hormone (PTH) resulted in the acceleration of injured nerve recovery. Proliferation of both
endoneurium and Schwann cells was observed on the 3rd day after the crushing of the ipsilateral extensor (N.
peroneus communis) and flexor (N. gastrocnemius) nerve bundles. A complete recovery of the AP enzymatic
activity was found on the 7–9th days after the crushing. From the 5th to 35th days after the crushing, we per-
formed online registration and mathematical analysis of pulse activity. In the control experiments, from the 35th
to 70th days, high-frequency stimulation of the nerves showed the regeneration of the extensor nerve only
accompanied by alterations in fiber direction. In the presence of PTH, the regeneration was significantly accel-
erated and it was associated with early regeneration of the flexor nerve and more structured fiber morphology.
We found a correlation between the electrophysiological and morphological data.
Keywords: nerve crushing, sciatic nerve, motoneurons, spinal cord, high frequency stimulation, extensor and
flexor collaterals, parathyroid hormone
DOI: 10.1134/S1819712410041026
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