Molecular Genetic Monitoring of Yersinia pseudotuberculosis
Using PCR O-Genotyping

V. T. Klimov and M. V. Chesnokova

Antiplague Research Institute of Siberia and the Far East, ul. Trilissera 78, Irkutsk, 664047 Russia

Received April 18, 2006

Abstract—PCR O-genotyping of 117 strains of Y. pseudotuberculosis, serotype O:1–O:4, isolated in Siberia
and in the Far East has been performed. This method allows identification of both O-genotype and its variants
(a, b, c). It has been shown that three O-genotypes of Y. pseudotuberculosis (O:1a, O:1b, and O:3) circulate in
Siberia and six types (O:1a, O:1b, O:1c, O:2a, O:3, and O:4b) circulate in the Far East. Genotype O:1b domi-
nates in both regions (87.8%). A PCR algorithm for identification of epidemically significant Y. pseudotuber-
culosis O-genotypes has been developed. The PCR analysis of clinical material makes it possible to identify the
O-genetic type of an etiological agent without bacteriological isolation of a pathogen.

DOI:10.3103/S0891416807040039

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