Molecular Genetic Techniques for Typing of Bartonella Isolates

M. Yu. Kirillov^a, A. P. Markov^a, I. V. Lopyrev^a, V. N. Pankratova ^a, S. A. Levitskii^a,
V. N. Bashkirov^a, G. B. Smirnov^a, A. N. Kruglov^b, V. A. Osadchaya^b, G. P. Frolova^b,
G. V. Barmina^b, O. A. Morozova^b, and M. Y. Kosoy^c

^a Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences,
ul. Gamalei 18, Moscow, 123098 Russia

^b Sechenov Moscow Medical Academy, ul. Trubetskaya 8, kor. 2, Moscow, 119992 Russia

^c Centers for Disease Control and Prevention, Fort Collins, CO, USA

Received December 28, 2005

Abstract—Primer sets for PCR detection of four housekeeping genes of Bartonella spp. isolated from clinical
material were developed and tested. We tested a strategy of the specific restriction fragment length polymor-
phism typing of bartonella species using as an example two strains isolated for the first time in Russia from
patients with endocarditis and fever of uncertain origin. The results of typing were confirmed by sequencing of
the obtained amplicons. The results of sequencing confirmed the affiliation of these isolates to the subspecies
B. vinsonii subsp. arupensis. The necessity of molecular epidemiological analysis of bartonellosis in Russia
was substantiated.

DOI:10.3103/S0891416807010028

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