of Thermophilic Bacterium Thermus ruber:
Cloning, Sequencing, and Properties
of Encoded
-Glutamylphosphate Kinase
S. Yu. Yaklichkin, M. S. Zimina, and L. V. Neumyvakin
Center of Thermobiotechnology and Molecular Diagnosis, Institute of Molecular Genetics,
Russian Academy of Sciences, Moscow, 123184 Russia
Received December 15, 1998
Abstract—The proB gene of the thermophilic bacterium Thermus ruber was cloned and sequenced, and sev-
eral properties of the encoded enzyme, 
-glutamylphosphate kinase (GPK), were studied. The proB open read-
ing frame was 1170 nt. Nucleotide sequence analysis revealed a GTG initiation codon at position 36 and a TGA
termination codon at position 1304. The gene had 68% GC, as characteristic of the genus Thermus. The
deduced protein product had a molecular mass of 41,984 Da. The ProB amino acid sequence showed the highest
homology (60%) to T. thermophilus GPK. The GPK activity was maximum (0.55 un./ml) at 55–65°C and weak
at 70°C. The enzyme can be used in biotechnological studies.
Key words: Thermus ruber, proB proline biosynthesis gene, thermophil, nucleotide sequence
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