Mouse Telomere-Binding Protein of Nuclear Matrix:
II. Localization

A. P. Voronin, I. B. Lobov, E. A. Bugaeva, V. N. Parfenov, and O. I. Podgornaya

Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064 Russia;
E-mail: podg@osint.stud.pu.ru

Received April 23, 1998; in final form, February 15, 1999

Abstract—Mouse matrix telomere-binding protein (mMTBP) was immunochemically stained with antibodies
against frog membrane telomere-binding protein (MTBP). In interphase nuclei, signals from mMTBP coin-
cided with those of FISH with a telomere-specific probe. In metaphase cells, weak signals were associated with
chromosomes, and more intense ones were beyond them. The latter proved to coincide with lamin B conglom-
erates; i.e., they were most probably associated with membrane vesicles, the remnants of the disintegrated
nuclear envelope. Signals from mMTBP were close to the nuclear envelope and coincided with some lamin B
signals in mouse liver interphase nuclei. Location of known telomere-binding proteins and the possible telom-
ere attachment to the nuclear envelope in normal cells are discussed.

Key words: telomere, telomere-binding protein, in situ DNA hybridization, immunohistochemistry

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