Telomere-Binding Activity of Fission Yeast
Schizosaccharomyces pombe
N. S. Vassetzky1, E. Gilson2, and S. M. Gasser3
1 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov St., Moscow, 117984 Russia;
E-mail: nvas@genome.eimb.relarn.ru
2 Ecole Normale Supérieure de Lyon, 46 allée d’Italie, 69364 Lyon Cedex 07, France
3 ISREC (Swiss Institute for Experimental Cancer Research), 155 Chemin de Boveresses,
CH-1066 Epalinges/Lausanne, Switzerland
Received April 17, 1998; in final form, November 19, 1998
Abstract—Band-shift assay reveals a telomeric factor in crude protein extracts of fission yeast Schizosaccha-
romyces pombe. This factor (SpTBP) specifically binds double-stranded DNA of the telomeric repeats of this
species; has lower and sequence-independent affinity to single-stranded DNA, and does not bind RNA. The bio-
chemical properties of SpTBP are discussed in the context of the authors' and literature data.
Key words: telomere; DNA–protein interaction; gel retardation assay
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