Transformation of Immortalized Rat Embryo Fibroblasts
with E7 Gene of Type 16 Human Papilloma Virus
V. A. Zhurbitskaya1, L. I. Fedorova2, N. M. Yartseva3, L. V. Savel’eva1,
A. V. Zelenin2, and F. L. Kisseljov1
1 Institute of Carcinogenesis, Blokhin Cancer Research Center, Russian Academy of Medical Sciences,
Moscow, 115478 Russia
2 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 117984 Russia
3 Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064 Russia
Received December 11, 1997; in final form, November 10, 1998
Abstract—The transforming properties of gene E7 of type 16 human papilloma virus (HPV16) transfected into
immortalized rat embryo fibroblasts were studied. Integration of E7 into the cell genome and its intracellular
expression were established. Its chromosomal localization was determined by in situ hybridization. Two cell
clones tr F8 and tr B4 differing in the phenotypic signs of transformation were obtained. The dependence of
proliferation of these cell clones on the growth factors and substrate, as well as contact inhibition of prolifera-
tion or topoinhibition were investigated. It was shown that differences in the biological characteristics of tr F8
and tr B4 did not correlate with the transforming gene expression intensity, no difference in the expression of
E7 mRNA was found.
Key words: human papilloma virus, HPV16, transforming genes, in situ hybridization, transformation
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