Yu. B. Shvarts1, E. S. Yudinkova2, S. A. Demakov1, S. V. Razin2, and I. F. Zhimulev1
1 Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090 Russia
2 Institute of Gene Biology, Russian Academy of Sciences, Moscow, 117334 Russia
Received June 15, 1998
Abstract—Nuclear matrix-associated regions (MAR) were studied in the DNA of interbands 61C7/C8,
85D9/D10, and 86B4/B6 from polytene chromosomes of Drosophila melanogaster. DNA fragments were radi-
olabeled at the ends and incubated in vitro with nuclear matrix preparation from Drosophila embryos. All inter-
band regions studied contained MAR. Some interbands probably correspond to the bases of the loops detected
biochemically in interphase chromosomes.
Key words: polytene chromosomes, interbands, nuclear matrix, in vitro binding
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