Analysis of Transcripts Specific for B-Cell
Non-Hodgkin Lymphomas in Patients Infected
with the Human Immunodeficiency Virus Type 1

A. I. Nikolaev¹, H. Hennig², I. A. Mukhoyan¹, V. V. Nenasheva¹, B. B. Kalmyrzaev¹,
V. I. Dubovaya¹, G. Hunsmann², V. Bodemer², and V. Z. Tarantul¹

¹ Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia
² German Primate Center, Goettingen, Germany

Received January 15, 1998

Abstract—Subtraction hybridization was used to obtain several cDNA clones which correspond to human
genes displaying enhanced expression upon lymphomogenesis in patients infected with HIV-1. Analysis of
cDNA specific for a centroblast non-Hodgkin lymphoma (NHL) revealed enhanced transcription of the
immunoglobulin light chain gene, the interferon-inducible gene, the gene for mitochondrial NADH dehydro-
genase 4, the set oncogene, and several unidentified genes. Several similar clones were detected in a library
obtained from another HIV-1 patient with immunoblast lymphoma. Nucleotide sequence analysis showed that
cDNA sequences homologous to set contain the set 3' noncoding region fused with the 3' sequence of the gene
for the constant region of 1 immunoglobulin light chain (1Ig). The fused sequence was detected in genomic
DNA of centroblast but not immunoblast lymphoma cells. A new chromosome translocation t(9;22)(q34;q11)
was assumed to occur upon lymphomogenesis in some HIV-1 patients.

Key words: lymphoma, human immunodeficiency virus, lymphoma-specific cDNAs, subtraction hybridization,
set oncogene, immunoglobulin chain gene, fused gene, human

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