Structure and Functions of the Erwinia herbicola Gene speA

S. V. Chernyshov, A. A. Mel’nikov, and Ya. I. Bur’yanov

Pushchino Branch, Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences,
Pushchino, Moscow Region, 142292 Russia

Received August 21, 1998

Abstract—Plasmid pAM28 obtained from Erwinia herbicola DNA suppresses the pathogenicity of E. caroto-
vora. Its origin was confirmed by hybridization with E. herbicola DNA. The speA (suppressing pathogenicity
of Erwinia) gene of pAM28 was localized. An open reading frame (ORF) coding for 166 amino acid residues
and regulatory elements (promoter regions –10 and –35 and the Shine–Dalgarno sequence) were found. Mutant
derivatives of pAM28 with deletions from the region of the SalI site did not suppress the pathogenicity of
E.carotovora. Sequencing showed that a mutant plasmid pF16 identical to pAM28 in molecular weight and
restriction sites but unable to suppress the pathogenicity of Erwinia had the speA identical to that of pAM28.
This suggests that pAM28 carries at least two genes responsible for the suppression of pathogenicity.

Key words: suppression of pathogenicity, Erwinia carotovora, DNA primary structure, speA gene

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