Protein Environment of Nucleotide U-1061
in the 790 StemLoop Region of 18S rRNA
in Human Ribosome 40S Subunit

A. A. Malygin, A. G. Veniyaminova, M. I. Dobrikov, V. N. Karavanov,
M. N. Repkova, G. V. Shishkin, and G. G. Karpova

Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences,
Novosibirsk, 630090 Russia

Received July 16, 1998

Abstract—We studied site-directed modification of 40S subunits of human ribosomes by derivatives of
oligodeoxyribonucleotide pON10 and oligoribonucleotide prON10 complementary to sites 1047–1061 and
10511061 of the 790 stem–loop region of 18S rRNA and carrying a photoactivatable group at the 5' terminus.
It was established that pON10 and prON10 can selectively bind to the address sequence in 40S subunits and, in
specific complexes, cross-link upon irradiation both with 18S rRNA and, preferentially, ribosomal proteins. The
cross-linking of both derivatives with 18S rRNA took place within a short site near to the 3'-terminal nucleotide
of the address sequence (U-1061). Proteins S2 and S3 proved to be the major targets of modification with the
prON10 derivative. A conclusion was drawn that these proteins are located in 40S subunits of human ribosomes
near to U-1061 of 18S rRNA.

Key words: human ribosome 40S subunits, DNA probe, mammalian ribosomal proteins S2 and S3, photocross-
linking

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