Interaction of Isolated -70 Subunit of RNA Polymerase
with Oligodeoxyribonucleotides Corresponding
to Regions 10 and 35 of the spc Promoter

I. I. Tulokhonov¹, L. K. Savinkova², V. A. Rau², and V. A. Rar¹

¹ Institute of Limnology, Siberian Division, Russian Academy of Sciences, Irkutsk, 664033 Russia
² Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090 Russia

Received March 27, 1998; in final form, November 18, 1998

Abstract—Binding of the isolated 70-kDa subunit (-70) of Escherichia coli RNA polymerase with oligode-
oxyribonucleotides (ON) corresponding to regions –10 and –35 of the spc promoter of E. coli ribosomal genes
was studied by gel filtration and gel retardation assay. The preparation of -70 was shown to contain no admix-
tures of DNA-binding proteins using 4-[N-methyl-N-(2-chloroethyl)amino]benzyl-5'-phosphamide derivatives
of ON.

Key words: subunit, Escherichia coli RNA polymerase, oligodeoxyribonucleotides, affinity

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