Extracting Autofluorescence Spectral Features for Diagnosis
of Nasopharyngeal Carcinoma¹

L. S. Lin^a, *, F. W. Yang^b, and S. S. Xie^a

^a Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology
for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China

^b Department of Otorhinolaryngology, Fuzhou General Hospital of the PLA, Fuzhou, China

*e-mail: lslin@fjnu.edu.cn

Received April 11, 2012; published online August 1, 2012

Abstract—The aim of this study is to investigate the autofluorescence spectral characteristics of normal and
cancerous nasopharyngeal tissues and to extract the potential spectral features for diagnosis of nasopharyngeal
carcinoma (NPC). The autofluorescence excitation-emission matrix (EEM) of 37 normal and 34 cancerous
nasopharyngeal tissues were recorded by a FLS920 spectrofluorimeter system in vitro. Based on the alteration
in proportions of collagen and NAD(P)H, the integrated fluorescence intensity of I_45510nm and I_38010nm were
used to calculated the ratio values by a two-peak ratio algorithm to diagnose NPC tissues at 340 nm excited.
Furthermore by applying the receiver operating characteristic curve (ROC), the 340 nm excitation yielded an
average sensitivity and specificity of 88.2 and 91.9%, respectively. These results may have practical implica-
tions for diagnosis of NPC.

DOI: 10.1134/S1054660X12090095

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