Fluorescence Spectra of Cultured Normal and Malignant Lung
Cells¹

M. Atif^{a, b,} *, M. S. AlSalhi^{a, c}, A. A. AlObiadi^c, and A. S. Aldwayyan^c

^a Research Chair for Laser Diagnosis of Cancer, Physics and Astronomy Department, College of Science,
King Saud University, Riyadh, Saudi Arabia

^bNational Institute of Lasers & Optronics, Islamabad, Pakistan

^cPhysics and Astronomy Department, College of Science, King Saud University, Riyadh, Saudi Arabia

*e-mail: atifhull@gmail.com

Received October 12, 2011; in final form, October 26, 2011; published online July 9, 2012

Abstract—Fluorescence Spectroscopy has emerged as a new modality to characterize physicochemical prop-
erties of biomolecules. The biomolecules have certain photophysical properties based on their molecular struc-
ture and these properties have been considered as useful parameters to monitor alterations in the functional,
morphological and micro environmental changes in the cells and tissues. In this study the fluorescence emission
spectra of normal and malignant lung cells were recorded for different excitation wavelengths: 230, 300, 340,
and 450 nm, corresponding to the absorption of tyrosine, tryptophan, collagen or elastin, Nicotinamide adenine
dinucleotide (NADH) and flavin adeno dinucleotide (FAD). Similarly excitation spectra were also recorded at
340 nm. The emission profiles showed considerable difference between the malignant and normal cells with the
malignant cells having more fluorescence intensity than that of normal cells keeping emission at 340 nm. Our
study had shown the discriminating features between normal and carcinoma cells lines because of higher con-
centration of tryptophan (1.5 times), NADH (3 times), and flavin (4 times) in carcinoma cell lines.

DOI: 10.1134/S1054660X1208004X

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