Optical Tweezers Technique for the Study
of Red Blood Cells Deformation Ability¹

A. Korobtsov^a, S. Kotova^a, *, N. Losevsky^a, A. Mayorova^a, V. Patlan^a,
E. Timchenko^a, N. Lysov^b, and E. Zarubina^b

^a Lebedev Physical Institute of Russian Academy of Sciences, Samara Branch, Samara, Russia

^b Samara Medical Institute REAVIZ, Samara, Russia

*e-mail: kotova@fian.smr.ru

Received February 24, 2012; published online June 12, 2012

Abstract—The presented here technique is developed for in vitro estimation of the influence of external con-
ditions on the deformation ability of human red blood cells. The method is based on the use of single-beam laser
trap for capturing of erythrocytes fixed to the bottom of the liquid cell by the adhesion force. The approach is
suitable for evaluation of the properties of individual cells as well as for real-time acquisition of the data on
ensemble of erythrocytes large enough for subsequent statistical analysis. It is shown that both a change in the
saline solution concentration and presence of certain stabilizing agents lead to measurable shift of the maximum
of the cells distribution by their deformability.

DOI: 10.1134/S1054660X12070067

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