Discrimination of NPC Cell Lines Associated with Malignant
Types Using Raman Spectroscopy¹

P. Liu^a, J. H. Li^a, Z. W. Tu^b, Z. Y. Guo^a, Z. F. Zhuang^a, *, and Y. F. Xia^b

^a MOE Key Laboratory of Laser Life Science & Laboratory of Photonic Chinese Medicine, College of Biophotonics,
South China Normal University, Guangzhou 510631, Guangdong, China

^b Department of Radiation Oncology, Cancer Center, Sun Yat-sen University of Medical Sciences,
Guangzhou 510060, China

*e-mail: zhuangzhf@yahoo.com.cn

Received May 27, 2012; in final form, June 20, 2012; published online October 1, 2012

Abstract—Nasopharyngeal carcinoma (NPC) is subdivided into four categories according to both the charac-
terization of the NPC cell and their radiotherapy reaction. To discriminate these kinds of NPC, the representa-
tive cultured cell types (CNE1, CNE2, 5-8F, and 6-10B) are explored by single-cell micro-Raman spectroscopy
(RS). The collected spectra are analyzed and compared by principal component analysis (PCA) and linear dis-
criminate analysis (LDA). The algorithm is shown to have the lowest specificity and sensitivity of 89and 92%
for NPC division. These results demonstrate that it is a potentially clinical application for Raman spectroscopy
to diagnosis NPC tissue types.

DOI: 10.1134/S1054660X12110096

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