Low Intensity Infrared Laser Effects on Escherichia coli Cultures
and Plasmid DNA¹

A. S. Fonseca^{a, b, c,} *, A. F. Teixeira^b, G. A. Presta^a, M. Geller^{c, d}, S. S. Valença^e, and F. Paoli^{c, f}

^a Departamento de Ciências Fisiológicas, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro,
Rua Frei Caneca, 94, Rio de Janeiro, 20211040, Brazil

^b Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes,
Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, Vila Isabel, Rio de Janeiro, 20551030, Brazil

^c Centro de Ciências da Saúde, Centro Universitário Serra dos Órgãos,
Avenida Alberto Torres, 111, Alto, Teresópolis, Rio de Janeiro, 25964004, Brazil

^d Setor de Facomatoses do Serviço de Genética Clínica do IPPMG, Universidade Federal do Rio de Janeiro,
Avenida Brigadeiro Trompowsky, Rio de Janeiro, 21949590, Brazil

^e Instituto de Ciências Biomédicas, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro,
Avenida Carlos Chagas Filho, 373, Bloco J, Ilha do Fundao, Rio de Janeiro, 21941902, Brazil

^f Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora,
Rua Jose Lourenço Kelmer, s/n—Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais 36036900, Brazil

*e-mail: adnfonseca@yahoo.com.br, adnfonseca@ig.com.br

Received September 22, 2011; in final form, September 26, 2011; published online September 3, 2012

Abstract—Biostimulative effect of low intensity laser in tissues has been described on a photobiological basis
and clinical protocols are recommended for treatment of various diseases. The aim of this work was to evaluate
effects of laser exposure on the survival of Escherichia coli cultures and plasmid topological forms. Escherichia
coli cultures and plasmids were exposed to infrared laser to study bacterial survival and electrophoretic profile,
respectively. Data indicate low intensity infrared laser: (i) had no effect on E. coli wild type, endonuclease IV,
exonuclease III, formamidopyrimidine DNA glycosylase/MutM protein and endonuclease III deficient cul-
tures, but decreased the survival of E. coli UvrA protein deficient cultures; (ii) there was no alteration in the
electrophoretic profile of plasmids. Exposure to low intensity infrared laser decreases survival of Escherichia
coli cultures deficient in nucleotide excision repair of DNA and this effect could depend on fluences, wave-
length and tissues conditions.

DOI: 10.1134/S1054660X12100076

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