Optical Parameters Measurement for Diagnostic
and Photodynamic Therapy of Human Cervical Adenocarcinoma
(HeLa) Cell Line¹

A. Rehman*, S. Firdous**, M. Nawaz***, and M. Ahmad****

Biophotonics Laboratory, National Institute of Lasers and Optronics, Nilore, Islamabad, 45650 Pakistan

*e-mail: aziz16pk@gmail.com

**e-mail: shamaraz@gmail.com

***e-mail: mnawaz@pinstech.org.pk

****e-mail: drmahmed@pieas.edu.pk

Received July 28, 2011; in final form, August 11, 2011; published online November 28, 2011

Abstract—The purpose of this study was to investigate the optical properties, absorption coefficient (_a) scat-
tering coefficient (_s) and refractive indices, (n) of HeLa cell line in a suspension of 2% minimum essential
medium (MEM) at two different (632.8 and 532.0 nm) wave lengths of laser light. Optical properties were
determined with Kubelka Munk Model (KMM) and refractive index measurement was made through minimum
angle of deviation method (MAD). We reported _a = 8.643 0.187 and 2.348 0.249 cm^–1 and _s = 5.609
0.287 and 88.166 2.833 cm^–1 at 632.8 and 532.0 nm, respectively. Refractive index was found to be 1.332 and
1.312 at 632.8 nm and 532.0 nm, respectively. The discussed results provide a route of information for clinical
diagnosis, therapeutic application and dosimetry studies in HeLa and other cell lines.

DOI: 10.1134/S1054660X12010161

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