Photodynamic Damage (PDD) Study
Using Stimulated Raman Scattering¹

M. S. AlSalhi^{a, b,} *, M. Atif^{a, b}, A. A. AlObiadi^a, and A. S. Aldwayyan^a

^a Physics and Astronomy Department, College of Science,
King Saud University, Riyadh, Saudi Arabia

^b KSU Research Chair for Laser Diagnosis of Cancer,
King Saud University, Riyadh, Saudi Arabia

*e-mail: malsalhy@gmail.com

Received February 7, 2011; in final form, July 6, 2011; published online November 28, 2011

Abstract—In the current study, photodynamic damage in different cell cultures was examined using a pulsed
laser light. Two different experiments were performed to analyse the photodynamic damage. For the first one,
a stimulated Raman scattering laser has been obtained by exciting DMSO liquid with Nd-YAG laser, second
harmonic generation, 532 nm. The resulted SRS wavelength is pulsed 630 nm. 1 ml ALA (200 g/ml) was
added to cell suspension and keep it for incubated for 4 h then irradiate the suspension with SRS wavelength
630 nm at different light dose 8, 12, 17, 25, 30, 40, 60, 100, 150, 200 J for 10 pluses and obtain the cell deg-
radation. We repeat the step above but for 30 pluses. Finally for the second experiment, 1 ml ALA (200 g/ml)
was added to cell suspension and was incubated for 4 h and then irradiated with Nd-YAG Laser at wavelength
532 nm. Different doses range between 8 up to 200 J for 10 pluses only and the cell degradation rate was mea-
sured.

DOI: 10.1134/S1054660X12010021

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