Blue Light-Activated Hypocrellin B Damages Ovarian
Cancer Cells¹

Y. Jiang^a, A. W. N. Leung^b, *, J. Y. Xiang^a, and C. S. Xu^{a, b,} * *

^a Department of Photodynamic and Sonodynamic Therapy, Second Affiliated Hospital, Chongqing Medical University,
Chongqing, China

^b School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China

*e-mail: xcshan@163.com
**e-mail: awnleung@cuhk.edu.hk

Received June 20, 2011; in final form, June 29, 2011; published online October 24, 2011

Abstract—In the present study, a novel blue light source from LED was used to activate hypocrellin B in ova-
rian cancer HO-8910 cells. Hypporellin B concentration was kept at 2.5 M and light doses from 0.5–4.0J/cm².
Photocytotoxicity was investigated using MTT reduction assay and light microscopy after light irradiation. Cel-
lular morphology was observed using transmission electron microscopy (TEM). MTT assay showed that the
cytotoxicity of blue light-activated hypocrellin B in HO-8910 cells increased along with light dose. The obser-
vations from light microscopy reinforced the above results. TEM showed that microvillin disappearance, vac-
uole formation, chromatin condensation, and topical apoptotic body were observed in the cells treated by both
light and hypocrellin B. The findings demonstrated that blue light from LED source could effectively activate
hypocrellin B to cause the destruction of HO-8910 cells, indicating that Blue light-activated hypocrellin B
might be potential therapeutic strategy in the management of ovarian cancer.

DOI: 10.1134/S1054660X11230071

Pleiades Publishing home page | journal home page | top

If you have any problems with this server, contact webmaster.