In Vitro Studies of Photofrin Mediated Photodynamic Therapy
on Human Rhabdomyosarcoma Cell Line (RD)¹

M. Atif^{a, b,} *, A. R. Malik^{b, c}, M. Fakhar-e-Alam^b, S. S. Hayat^c,
S. S. Z. Zaidi^d, R. Suleman^d, and M. Ikram^b

^a Research Chair for Laser Diagnosis of Cancer, Physics and Astronomy Department, College of Science,
King Saud University, Riyadh, Saudi Arabia

^b National Institute of Laser and Optronics, Nilore, Islamabad, 45650 Pakistan

^c Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, 45650 Pakistan

^d Hazara University Mansehra, KPK, Pakistan

^e National Institute of Health, Pakistan

*e-mail: atifhull@gmail.com

Received June 14, 2011; in final form, June 19, 2011

Abstract—In the current study, the cytotoxic effects induced by the given photosensitizer (PS) on human rhyb-
domyosarcoma cancer cells (RD) as an experimental model were investigated. The experimental results like
cytotoxic effects induced by the given PS on RD cells were dose dependent and the optimum concentration of
Photofrin^® (100 g/ml) along with 120 J/cm² generates the maximum loss in cell viability which is almost 82%.
The significant loss in cell viability is the result of interaction of suitable dose of laser light (630nm of wave-
length) with Photofrin^®, after excitation of PS, production of reactive oxygen species (ROS), which leads to
mitochondria damage and resulting of cell death (cell necrosis/cell apoptosis). Viability of controlled and
treated RD cells with optimum dose of light (630 nm) has been assessed by neutral red assay (NRA) and cell
damaging effect were verified by staining of mitochondria using Mitotracker^® red as an efficient dye as well as
reactive oxygen species (ROS) accumulation detection.

10.1134/S1054660X11230010

Pleiades Publishing home page | journal home page | top

If you have any problems with this server, contact webmaster.