Measurement of Metabolites of Polycyclic Aromatic Hydrocarbons
in Fish Bile for Comparative Assessment of Coastal Water Pollution
N. N. Belchevaa, *, A. A. Istominaa, b, A. Yu. Zvyagintsevc,
I. V. Epurc, A. A. Karpenkoc, and E. P. Karaulovad
Translated by S. Avodkova
aIl’ichev Pacific Oceanological Institute, Far East Branch, Russian Academy of Sciences (POI FEB RAS), Vladivostok, Russia
bFar Eastern State Technical Fisheries University, Vladivostok, Russia
cZhirmunsky Institute of Marine Biology, Far East Branch, Russian Academy of Sciences (IMB FEB RAS), Vladivostok, Russia
dPacific Scientific Research Fisheries Center (PSRF Center), Vladivostok, Russia
Correspondence to: *e-mail: belcheva@poi.dvo.ru
Received 5 July, 2016
Abstract—In the laboratory experiment and in fish caught in conditionally clean and polluted areas, the presence of metabolites of polycyclic aromatic hydrocarbons in bile by methods of conventional and synchronous spectrofluorimetry was determined. The study showed that this approach is well suited for the detection of metabolites of polycyclic aromatic hydrocarbons with 2–3, 4, and 5–6 aromatic rings. If the content of metabolites of polycyclic aromatic hydrocarbons with 4 and 5–6 rings in bile is low, the method of synchronous spectrofluorimetry is less sensitive than conventional spectrofluorimetry. Both methods of monitoring are simple and fast and reflect the real picture of water pollution.
Keywords: bile of fish, polycyclic aromatic hydrocarbons, biomonitoring, fixed wavelength and synchronous fluorescence
DOI: 10.1134/S0032945217050034