Synthesis and In vitro Study of Cytotoxic Activity of New Tetrazole-Containing 2,4-Diamino-1,3,5-triazine Derivatives

O. V. Mikolaichuk^{a, b, c}^,*, A. V. Protas^{a, c}, E. A. Popova^{a, c}, O. E. Molchanov^c, D. N. Maistrenko^c, V. A. Ostrovskii^d, Yu. N. Pavlyukova^d, V. V. Sharoyko^{a, b, c}, and K. N. Semenov^{a, b, c}

^aI.P. Pavlov First St. Petersburg State Medical University, St. Petersburg, 197022 Russia

^bSt. Petersburg State University, St. Petersburg, 199034 Russia

^cA. M. Granov Russian Scientific Center of Radiology and Surgical Technologies, St. Petersburg, 197758 Russia

^dSt. Petersburg State Institute of Technology (Technical University), St. Petersburg, 190013 Russia

email: *olga19940519@mail.ru

Received 30 June, 2022

Abstract— New tetrazole-containing derivatives of 2,4-diamino-1,3,5-triazine were synthesized. The cytotoxic activity of the obtained compounds against Huh-7 and HeLa tumor cell lines was studied by the MTT test. The most significant antitumor activity was shown by 1,3,5-triazines containing a 5-phenyltetrazol-2-ylacetohydrazide fragment. The resulting compounds showed practically no cytotoxic effect on non-tumor cells of the human embryonic kidney line HEK293.

Keywords: 1,3,5-triazines, tetrazoles, MTT test, cytotoxic activity

DOI: 10.1134/S1070363222090055