E. V. Akhidovaa, c, 1, T. D. Volkovaa, D. O. Koroeva, I. Yu. Yakupova, M. V. Kalintsevaa,
L. E. Zavalishinab, A. P. Kaplunc, O. O. Zharskayaa, O. V. Zatsepinaa, and O. M. Volpinaa
a Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences,
ul. Miklukho-Maklaya, 16/10, Moscow, 117997, Russia
b Herzen Cancer Research Institute, Moscow, Russia
c Lomonosov State University of Fine Chemical Technologies, Moscow, Russia
Received September 21, 2012; in final form, October 26, 2012
Abstract—Tumor-associated protein survivin is a bifunctional protein that can participate either in the regula-
tion of cell division or in the inhibition of apoptosis depending on its location in the cell and its structural state.
The aim of the present work was to obtain monospecific antibodies to survivin targeting various sites in the pro-
tein and suitable for the study of its structural and functional features. The ability of the antibodies to detect
survivin in tumor cells and mammary tumor tissues has been assessed. Antibodies targeting the (1–22) and (95–
105) fragments of the protein have been shown to have the highest specific activity. Antibodies targeting the (1–
22) site preferentially bound to survivin-containing complex (presumably a dimer of survivin) in immunoblot-
ting, whereas antibodies targeting the (95–105) site detected only the monomeric form of the protein. Analysis
of malignant mammary tumor samples showed that monomeric survivin was present only in tumor tissue sam-
ples, whereas survivin-containing complex was expressed both in tumor tissues and in the tissues adjacent to
the tumor. Antibodies targeting the (1–22) site were shown to preferentially detect survivin localized in the cell
nuclei (and involved in the regulation of mitosis) in immunocytochemistry, while those targeting the (95–105)
site stained the nucleoplasm and cytoplasm at all stages of the cell cycle. Therefore, the antibodies obtained can
serve as a useful tool for structural and functional research on survivin.
Keywords: survivin, synthetic peptides, antibodies, survivin-containing complex
DOI: 10.1134/S1068162013030023
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