M. E. Palkeeva1, M. V. Sidorova, A. S. Molokoedov, A. A. Az’muko, Zh. D. Bespalova, T. V. Sharf,
E. N. Mamochkina, E. E. Efremov, M. M. Rogova, N. A. Mironova, K. A. Zykov, and S. P. Golitsyn
Russian Cardiological Research and Production Complex, Russian Ministry of Public Health,
ul. 3-ya Cherepkovskaya 15a, Moscow, 121552 Russia
Received October 19, 2012; in final form, December 26, 2012
Abstract—Fragments corresponding to the 83–98 sequence of the first extracellular loop and to the 168–192
and 171–182 sequences of the second extracellular loop of the M2-muscarinic receptor (antibodies to this
receptor could be markers of early symptoms of heart disorders) were synthesized by solid phase method using
the Fmoc-SPPS strategy. A new conformational antigen with the natural location of the disulfide bridge was
prepared by selective formation of disulfide bond between the corresponding cysteine residues in the synthe-
sized peptides and characterized. The comparative analysis of reactivity of the synthesized peptides towards
sera from patients which had no organic heart disease was performed. A new conformational antigen was effec-
tively bound to the sera from patients with idiopathic arrhythmias, but without symptoms of organic heart dis-
ease.
Keywords: idiopathic arrhythmia, antibodies to M2-muscarinic receptor, conformational peptide antigens, syn-
thetic peptides, selective disulfide bond formation, solid phase synthesis
DOI: 10.1134/S1068162013030102
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