Phenothiazine Derivatives as Enhancers
of Peroxidase-Dependent Chemiluminescence

M. M. Vdovenko, A. Kh. Vorobiev, and I. Yu. Sakharov¹

Chemistry Department, Moscow State University, Moscow, 119991 Russia

Received May 10, 2012; in final form, May 21, 2012.

Abstract—Some N-alkyl phenothiazines with different ionic groups were studied as enhancers of chemilumi-
nescence catalyzed by soybean peroxidase. It was shown that under experimental conditions, the compounds
with positively charged groups do not exhibit enhancing ability, while the addition of phenothiazines with neg-
atively charged groups to a substrate mixture significantly increased the chemiluminescence intensity. The rela-
tionship between the enhancing activity of phenothiazines and their capacity for enzymatic oxidation by hydro-
gen peroxide was found. The enhancers discovered new opportunities for increasing the sensitivity of determi-
nation of analytes by chemiluminescent enzyme immunoassay.

Keywords: phenothiazine, peroxidase, chemiluminescence, enhancers

DOI: 10.1134/S1068162013020155

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