A. A. Stakheev, D. R. Khairulina, D. Yu. Ryazantsev, and S. K. Zavriev1
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences,
ul. Miklukho-Maklaya 16/10, Moscow, 117997 Russia
Received October 1, 2012; in final form, October 15, 2012
Abstract—A PCR-based system has been developed for highly specific identification of the phytopathogenic
fungus Fusarium cerealis on the basis of the nucleotide sequence of the phosphate permease gene. Sequencing
and the following analysis showed that this gene demonstrated a high degree of polymorphism and can be used
for both phylogenetic studies and as a marker for specific primer design. Investigation of the specificity of the
designed primers confirmed the lack of cross-reactivity with DNA of closely related fungi species of the Fusar-
ium genus. The qPCR assay demonstrated high sensitivity of the test system, which was 10pg of the specific
DNA per reaction.
Keywords: Fusarium cerealis, DNA markers, PCR identification, phosphate permease gene
DOI: 10.1134/S1068162013020131
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