TUBB3 Expression in Nonsmall Cell Lung Cancer Tissue: Reasons for Immunohistochemical Assay Discordance and an Approach to Overcome It

T. A. Bogush^a^,*, A. Lee^a, S. A. Kalyuzhny^a, E. A. Bogush^b, M. Ya. Melnikov^c, and V. S. Kosorukov^a

^aBlokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, 115522 Russia

^bFirst Sechenov Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, 119435 Russia

^cMoscow State University, Moscow, 119991 Russia

email: *tatbogush@mail.ru

Received 25 May, 2024

Abstract— The reasons for discordance data on the contribution of a TUBB3 microtubule protein to resistance to antitumor drugs and the metastatic potential of tumors with an assessment of prognostic significance of TUBB3 expression in nonsmall cell lung cancer tissue are analyzed. The need to introduce quantitative analysis methods in molecular diagnostics of tumors (in particular, immunofluorescence assessment associated with flow cytometry) is substantiated.

Keywords: TUBB3, nonsmall cell lung cancer, immunofluorescence analysis, flow cytometry, immunohistochemical analysis

DOI: 10.3103/S0027131424700457