Biopolymer Controlled Release Systems Based on Hydrolyzed Collagen: Cryoforming, Structure, and Properties

Abstract— Biopolymer materials based on natural collagen (gelatin, hydrolyzed collagen) are widely used in the food, pharmaceutical, and cosmetic industries due to their low toxicity, high biocompatibility, low antigenicity, and unique mechanical and technological properties. Hydrolyzed collagen, unlike gelatin, is formed by peptides with a lower molecular weight. Its advantages are higher bioavailability and biodegradability in comparison with gelatin. In this study, biopolymer matrices containing a dioxidine antibacterial drug are obtained based on hydrolyzed collagen using low-temperature technologies. It is shown that, varying synthesis parameters such as the concentration of hydrolyzed collagen in the precursor solution (from 1 to 10%), matrix crosslinking time (0.1–24 h), and cryoforming temperature (–30 and –196°C), it is possible to change the morphology and structure of the matrix, its degradation time, and drug release time. The composition and structure of dioxidine/hydrolyzed collagen systems are characterized by SEM and IR and UV spectroscopy. The antibacterial activity of the resulting dioxidine/hydrolyzed collagen systems against E. coli and S. aureus is characterized by the disk diffusion method.