Protein Engineering of Bst Polymerase for Isothermal Amplification Purposes

A. S. Cherkashina^a^, *, O. O. Mikheeva^a, and V. G. Akimkin^a

^aCentral Research Institute of Epidemiology of Rospotrebnadzor, Moscow, 111123 Russia

Correspondence to: *e-mail: cherkashina@pcr.ms

Received 15 November, 2023

Abstract— This paper reviews the protein engineering of Bst polymerase using various methods. To modify the enzyme, approaches such as the production of chimeric proteins, directed evolution, and directed and random mutagenesis are used. Examples of successful changes in enzyme properties such as catalytic activity, processivity, thermal stability, and resistance to inhibitors are described.

Keywords: Bst-polymerase, large fragment, strand displacement activity, isothermal amplification, mutagenesis

DOI: 10.3103/S002713142470007X