Age-Related Changes in the Expression of Lamin B Receptors in Human Dermal Fibroblasts

Abstract—The goal of this research was to study the content of lamin B receptors in human dermal fibroblasts during different age periods (from a 20-week pregnancy to the age of 85) in correlation with their proliferative activity. Lamin B receptors and proliferating cell nuclear antigen (PCNA) were identified in skin sections by indirect immunohistochemistry. Our results have shown that an increase in the share of fibroblasts with positive staining for the lamin B receptors and an increase in the expression level of lamin B receptors in fibroblast nuclei are observed during the period from age zero to 20 years of age as compared with other age periods. The total fibroblast count, including positively PCNA-stained dermal fibroblasts, decreased most significantly from the age of 20. The increase in the content of lamin B receptors in dermal fibroblasts, which is observed from birth until the age of 20, can be considered one of the triggering moments leading to the age-dependent decrease in counts of human dermal fibroblasts.